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Antihypertensive Effects of Peptides in Autolysate of Bonito Bowels on Spontaneously Hypertensive Rats

1993; Oxford University Press; Volume: 57; Issue: 12 Linguagem: Inglês

10.1271/bbb.57.2186

1347-6947

Mikio Fujii, Nobuyasu Matsumura, Kazue Mito, Toshio Shimizu, Masayoshi Kuwahara, Shigeru SUGANO, Hideaki Karaki,

Muscle metabolism and nutrition

An autolysate of bonito bowels was treated with ultrafiltration, loose RO concentration, ion-exchange chromatography, and reverse phase chromatography to increase its potency to inhibit angiotensin I-converting enzyme (ACE) activity by 16-fold. Oral administration of the partially purified autolysate decreased the systolic blood pressure of spontaneously hypertensive rats (SHR) in a dose-dependent manner at the doses of 1 g peptides/kg or higher. The relationship between the antihypertensive activity (in vivo) of the partially purified preparation and its ACE inhibitory activity (in vitro) in comparison with previously reported ACE inhibitory peptides is discussed.