Hydrolysis of 4‐methylumbelliferyl butyrate: A convenient and sensitive fluorescent assay for lipase activity
1985; Wiley; Volume: 20; Issue: 4 Linguagem: Inglês
10.1007/bf02534195
ISSN1558-9307
Autores Tópico(s)Electrochemical sensors and biosensors
ResumoAbstract A rapid, sensitive and convenient fluorescent assay was developed to screen for lipase activity. The non‐fluorescent substrate, 4‐methylumbelliferyl butyrate (4‐MUB), solubilized in either liposomal dispersions or bile salt/lecithin mixed micelles, is hydrolyzed to butyric acid and the highly fluorescent compound, 4‐methylumbelliferone (4‐MU). Assays are run at 37 C for 10 min, terminated, and the changes in fluorescence quantitated with a Turner III fluorometer. Both lingual and pancreatic lipases exhibit activity against this artificial substrate. The assay has several advantages: nmoles 4 MU/ml/hr are measured allowing the detection of very low lipolytic activity; multiple samples may be simultaneously assayed, and only a brief incubation period is required.
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