Artigo Acesso aberto Revisado por pares

Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

1984; Elsevier BV; Volume: 73; Issue: 1 Linguagem: Inglês

10.1016/0022-1759(84)90033-4

ISSN

1872-7905

Autores

M Stya, Richard L. Wahl, William H. Beierwaltes,

Tópico(s)

Viral Infectious Diseases and Gene Expression in Insects

Resumo

Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.

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