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In vitro inhibition of antirecoverin immunoglobulin-mediated death of mammalian photoreceptor cells

2001; Wiley; Volume: 63; Issue: 2 Linguagem: Inglês

10.1002/1097-4547(20010115)63

1097-4547

Weiheng Chen, Wei Cao, Ann Mary Achyuthan, James F. McGinnis,

Receptor Mechanisms and Signaling

Journal of Neuroscience ResearchVolume 63, Issue 2 p. 116-123 Research Article In vitro inhibition of antirecoverin immunoglobulin-mediated death of mammalian photoreceptor cells Weiheng Chen, Weiheng Chen Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CitySearch for more papers by this authorWei Cao, Wei Cao Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CitySearch for more papers by this authorAnn Mary Achyuthan, Ann Mary Achyuthan Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CitySearch for more papers by this authorJames F. McGinnis, Corresponding Author James F. McGinnis james-mcginnis@ouhsc.edu Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CityDepartment of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma, 608 Stanton Young Blvd, Oklahoma City, OK 73104Search for more papers by this author Weiheng Chen, Weiheng Chen Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CitySearch for more papers by this authorWei Cao, Wei Cao Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CitySearch for more papers by this authorAnn Mary Achyuthan, Ann Mary Achyuthan Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CitySearch for more papers by this authorJames F. McGinnis, Corresponding Author James F. McGinnis james-mcginnis@ouhsc.edu Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma CityDepartment of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma, 608 Stanton Young Blvd, Oklahoma City, OK 73104Search for more papers by this author First published: 12 January 2001 https://doi.org/10.1002/1097-4547(20010115)63:2 3.0.CO;2-GCitations: 6Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Cancer-associated retinopathy (CAR) is a blinding disease, which can be mediated by autoimmune reactions with a specific calcium-binding retinal protein, recoverin. A number of recent studies demonstrate that agents that mobilize intracellular calcium can protect neurons from apoptotic death induced by a variety of insults. In this study, we investigated the effect of one such agent, potassium, on the survival of mammalian rod photoreceptors exposed to antirecoverin IgG. Primary cell cultures of rat retinal neurons were grown in a chemically defined medium, and cells were exposed to antirecoverin IgG for 72 hr in various concentrations of potassium and the surviving cells counted. Rod photoreceptors were quantitated using antirhodopsin immunofluorescence microscopy, and total cell numbers were determined by 4′,6-diamidino-2-phenylindole (DAPI) staining of nuclei. Apoptosis was evaluated by TdT-mediated biotin-dUTP nick-end labeling (TUNEL), cell death-detection ELISA, and DNA laddering. The present study shows that elevated extracellular K+ ([K+]o) protects retinal neurons from antirecoverin antibody-mediated cell death. The protective effects of [K+]o were shown to be time- and dose-dependent. The inhibition of antirecoverin IgG-mediated death of photoreceptors by elevated [K+]o suggests that the mobilization of internal calcium stores rescues the cells by interfering with apoptotic signal transduction pathways. These data also suggest that the death of photoreceptor cells occurring in CAR possibly can be prevented by reagents and/or environmental changes that mobilize intracellular calcium. J. Neurosci. Res. 63:116–123, 2001. © 2001 Wiley-Liss, Inc. Citing Literature Volume63, Issue215 January 2001Pages 116-123 RelatedInformation