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Epidermal growth factor induces cell cycle arrest and apoptosis of squamous carcinoma cells through reduction of cell adhesion

2000; Wiley; Volume: 77; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-4644(20000615)77

1097-4644

Liu Cao, Yeqi Yao, Vivian S. Lee, Chris Kiani, David Spaner, Zhaosheng Lin, Yaou Zhang, Mark E. Adams, Burton B. Yang,

Cellular Mechanics and Interactions

Journal of Cellular BiochemistryVolume 77, Issue 4 p. 569-583 Article Epidermal growth factor induces cell cycle arrest and apoptosis of squamous carcinoma cells through reduction of cell adhesion Liu Cao, Liu Cao Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada Liu Cao and Yeqi Yao contributed equally to this study.Search for more papers by this authorYeqi Yao, Yeqi Yao Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada Liu Cao and Yeqi Yao contributed equally to this study.Search for more papers by this authorVivian Lee, Vivian Lee Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorChris Kiani, Chris Kiani Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorDavid Spaner, David Spaner Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, CanadaSearch for more papers by this authorZhaosheng Lin, Zhaosheng Lin Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorYaou Zhang, Yaou Zhang Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorMark E. Adams, Mark E. Adams Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorBurton B. Yang, Corresponding Author Burton B. Yang byang@srcl.sunnybrook.utoronto.ca Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaResearch Building, Sunnybrook and Women's College Health Sciences Centre, 2075 Bayview Avenue, Toronto M4N 3M5 CanadaSearch for more papers by this author Liu Cao, Liu Cao Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada Liu Cao and Yeqi Yao contributed equally to this study.Search for more papers by this authorYeqi Yao, Yeqi Yao Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada Liu Cao and Yeqi Yao contributed equally to this study.Search for more papers by this authorVivian Lee, Vivian Lee Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorChris Kiani, Chris Kiani Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorDavid Spaner, David Spaner Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, CanadaSearch for more papers by this authorZhaosheng Lin, Zhaosheng Lin Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorYaou Zhang, Yaou Zhang Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorMark E. Adams, Mark E. Adams Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaSearch for more papers by this authorBurton B. Yang, Corresponding Author Burton B. Yang byang@srcl.sunnybrook.utoronto.ca Sunnybrook and Women's College Health Sciences Centre, University of Toronto, Toronto, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, CanadaResearch Building, Sunnybrook and Women's College Health Sciences Centre, 2075 Bayview Avenue, Toronto M4N 3M5 CanadaSearch for more papers by this author First published: 14 April 2000 https://doi.org/10.1002/(SICI)1097-4644(20000615)77:4 3.0.CO;2-KCitations: 31 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Most squamous epithelial cells are strictly anchorage-dependent cell types. We observed that epidermal growth factor (EGF) promoted the growth of A431 squamous carcinoma cells in suspension cultures but suppressed cell growth and induced apoptosis in monolayer cultures, suggesting that loss of adhesion is responsible for the effects observed in monolayer culture, before cell death. Consistent with this finding, we demonstrated that EGF reduced cell attachment, cell-cell interaction, and cell spreading. Treatment with EGF increased cell adhesion-regulated expression of p21 but suppressed expressions of cyclin A, D1, cdk2, and retinoblastoma protein (pRb), leading to cell cycle arrest and adhesion-regulated programmed cell death. To test directly whether promoting cell adhesion could reduce the effects of EGF, we grew cultures on plates coated with type II collagen. On these plates, cell adhesion was enhanced and EGF treatment had little effect on cell adhesion and apoptosis when cells were attached to the collagen. The collagen effects were dose dependent, and cell cycle and cell cycle-associated proteins were altered accordingly. Finally, when cultures were plated on bacterial Petri dishes, which completely disrupted cell attachment to substratum, the level of apoptosis was greatly higher and cell cycle was arrested as compared with monolayer cultures. Taken together, our results strongly suggest that the EGF-induced cell cycle arrest and apoptosis in monolayer cultures was the result of a decline in cell adhesion. J. Cell. Biochem. 77:569–583, 2000. © 2000 Wiley-Liss, Inc. Citing Literature Volume77, Issue415 June 2000Pages 569-583 RelatedInformation