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Total sulphur amino acid requirement and maximum cysteine replacement value for methionine for fingerling Catla catla (Hamilton)

2014; Wiley; Volume: 47; Issue: 1 Linguagem: Inglês

10.1111/are.12493

1365-2109

Seemab Zehra, Mukhtar A. Khan,

Reproductive biology and impacts on aquatic species

Aquaculture ResearchVolume 47, Issue 1 p. 304-317 Original Article Total sulphur amino acid requirement and maximum cysteine replacement value for methionine for fingerling Catla catla (Hamilton) Seemab Zehra, Seemab Zehra Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, IndiaSearch for more papers by this authorMukhtar A Khan, Corresponding Author Mukhtar A Khan Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, IndiaCorrespondence: M A Khan, Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202 002, India. E-mail: khanmukhtar@yahoo.comSearch for more papers by this author Seemab Zehra, Seemab Zehra Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, IndiaSearch for more papers by this authorMukhtar A Khan, Corresponding Author Mukhtar A Khan Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, IndiaCorrespondence: M A Khan, Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202 002, India. E-mail: khanmukhtar@yahoo.comSearch for more papers by this author First published: 02 June 2014 https://doi.org/10.1111/are.12493Citations: 9Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Two separate 12 weeks feeding trials were performed to quantify the total sulphur amino acid (TSAA) requirement (experiment I) and cysteine replacement value for methionine (experiment II) of fingerling Catla catla. In experiment I, six casein–gelatin based (33% crude protein; 16.72 kJ g−1 gross energy) diets with graded levels of TSAA (0.56%, 0.81%, 1.06%, 1.31%, 1.56%, 1.81% dry diet) were fed to triplicate groups of fish (3.55 ± 0.06 cm; 0.65 ± 0.02 g) near to satiation. The TSAA requirement was determined by quadratic regression analysis of absolute weight gain (AWG), protein efficiency ratio (PER), feed efficiency (FE), protein gain (PG) and TSAA gain (TSAAG) against dietary TSAA concentrations at 95% maximum response. Above analysis revealed that inclusion of TSAA at 1.28% dry diet (1.22% methionine + 0.06% cysteine), corresponding to 3.87% of dietary protein is optimum. In experiment II, to determine the replacement value of cysteine for methionine, six diets containing 1.28% TSAA determined in experiment I with different ratios of l-methionine and l-cysteine (80:20, 70:30, 60:40, 50:50, 40:60, 30:70) on equimolar sulphur basis were fed to fish (3.65 ± 0.08 cm; 0.67 ± 0.04 g). Broken-line regression analysis of AWG, PER, FE, PG and TSAAG against varying methionine to cysteine ratios yielded the optimum cysteine replacement value at 40.3%. Based on above analysis, it is recommended that inclusion of 1.28% dietary TSAA, corresponding to 3.87% of dietary protein is optimum of which 40.3% could be spared by cysteine. Data generated during this study would be useful in formulating TSAA balanced, cost-effective feeds for the intensive culture of this fish. Citing Literature Volume47, Issue1January 2016Pages 304-317 RelatedInformation