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REPEATED PGE1 TREATMENT ENHANCES NITRIC OXIDE AND ERECTION RESPONSES TO NERVE STIMULATION IN THE RAT PENIS BY UPREGULATING CONSTITUTIVE NOS ISOFORMS

1999; Lippincott Williams & Wilkins; Volume: 162; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(05)68160-8

1527-3792

Ana Escrig, Raquel Marín, Manuel Mas,

Urinary Bladder and Prostate Research

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Dec 1999REPEATED PGE1 TREATMENT ENHANCES NITRIC OXIDE AND ERECTION RESPONSES TO NERVE STIMULATION IN THE RAT PENIS BY UPREGULATING CONSTITUTIVE NOS ISOFORMS ANA ESCRIG, RAQUEL MARIN, and MANUEL MAS ANA ESCRIGANA ESCRIG , RAQUEL MARINRAQUEL MARIN , and MANUEL MASMANUEL MAS View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)68160-8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To assess whether intracavernosal injections of prostaglandin E1 (PGE1) can influence nitric oxide (NO) release in the corpora in a rat model of penile erection. Materials and Methods: The extracellular levels of NO were monitored at 100 seconds intervals in the corpus cavernosum of anesthetized rats by using differential normal pulse voltammetry with porphyrin-Nafion coated carbon fiber microelectrodes. The intracavernosal pressure (ICP) was simultaneously recorded. PGE1 was given either as a single dose (ranging from 0.2 to 15 μg.) or as repeated 2 μg. injections in alternate days for two weeks. The NO and ICP responses to electrostimulation of the cavernosal nerve (SCN) was studied in the animals in the repeated treatment schedule at 1, 7, 15 and 30 days after its termination. The levels of the three NO synthase (NOS) isoforms in the cavernous tissue were measured by immunoblotting. Results: Acute PGE1 treatment dose-relatedly increased NO levels in the corpora, with a concomitant ICP increase with the highest dose. Repeated 2 μg. PGE1 injections increased the NO and ICP responses to SCN as compared with intact or vehicle-injected animals. This treatment also increased the penile content of the neuronal and endothelial NOS proteins. The inducible NOS isoform remained unchanged after either vehicle or PGE1 injections. 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Google Scholar From the University of La Laguna, School of Medicine, Department of Physiology, 38071 Tenerife, Spain.© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byRUBIO J, HERNÁNDEZ M, ARCOS L, MARTÍNEZ A, GARCÍA-SACRISTÁN A and PRIETO D (2018) Mechanisms of Prostaglandin E1-Induced Relaxation in Penile Resistance ArteriesJournal of Urology, VOL. 171, NO. 2, (968-973), Online publication date: 1-Feb-2004. Volume 162Issue 6December 1999Page: 2205-2210 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.Keywordsnitric oxidepenile erectionnitric oxide synthaseprostaglandin E1MetricsAuthor Information ANA ESCRIG More articles by this author RAQUEL MARIN More articles by this author MANUEL MAS More articles by this author Expand All Advertisement PDF downloadLoading ...