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Simultaneous Detection and Identification of Enteric Viruses by PCR-Mass Assay

2012; Public Library of Science; Volume: 7; Issue: 8 Linguagem: Inglês

10.1371/journal.pone.0042251

1932-6203

Jingzi Piao, Jun Jiang, Bianli Xu, Xiaohong Wang, Yanfang Guan, Weili Wu, Licheng Liu, Yuan Zhang, Xueyong Huang, Pengzhi Wang, Jinyin Zhao, Xiaoping Kang, Hua Jiang, Cao Yuan-yin, Yuling Zheng, Yongqiang Jiang, Yan Li, Yinhui Yang, Weijun Chen,

Herpesvirus Infections and Treatments

Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simultaneous detection of eight distinct human enteric viruses. Enteric viral isolates and standard viral RNAs were examined to determine the sensitivity and specificity of the PCR-Mass assay. Clinical performance was evaluated with a total of 101 clinical specimens from patients suspected of having hand, foot and mouth disease (HFMD). The results were compared to those of previous analyses using real-time RT-PCR. The identification of specific viruses and clinical specimens shows that the PCR-Mass assay performed as well as or better than standard methods with respect to indicating the presence of multiplex pathogens in a single specimen.