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Effect of nerve crush on perikaryal number and volume of neurons in adult rat dorsal root ganglion

1999; Wiley; Volume: 412; Issue: 1 Linguagem: Inglês

10.1002/(sici)1096-9861(19990913)412

1096-9861

J. Degn, Trine Tandrup, Johannes Jakobsen,

Retinoids in leukemia and cellular processes

Journal of Comparative NeurologyVolume 412, Issue 1 p. 186-192 Effect of nerve crush on perikaryal number and volume of neurons in adult rat dorsal root ganglion J. Degn, Corresponding Author J. Degn [email protected] Department of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, DenmarkDepartment of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark.Search for more papers by this authorT. Tandrup, T. Tandrup Department of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark Stereological Research Laboratory, University of Aarhus, DK-8000 Aarhus C, DenmarkSearch for more papers by this authorJ. Jakobsen, J. Jakobsen Department of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, DenmarkSearch for more papers by this author J. Degn, Corresponding Author J. Degn [email protected] Department of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, DenmarkDepartment of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark.Search for more papers by this authorT. Tandrup, T. Tandrup Department of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark Stereological Research Laboratory, University of Aarhus, DK-8000 Aarhus C, DenmarkSearch for more papers by this authorJ. Jakobsen, J. Jakobsen Department of Neurology, University Hospital of Aarhus, DK-8000 Aarhus C, DenmarkSearch for more papers by this author First published: 28 July 1999 https://doi.org/10.1002/(SICI)1096-9861(19990913)412:1 3.0.CO;2-HCitations: 33AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Assumption-free stereological methods were applied to assess the effect of nerve crush on perikaryal number and mean volume of neuronal subpopulations in adult rat dorsal root ganglion (DRG). The L5 spinal nerve of 20 Wistar rats was crushed approximately 7 mm distal to the DRG, and the contralateral spinal nerve and DRG were left intact and used as controls. After four, 15, 45, and 120 days, the rats were killed, and the tissue was fixed and processed for subsequent preparation of 30-μm-thick sections. Estimates of neuron number were obtained with the optical fractionator technique and estimates of the mean perikaryal volume with the vertical planar rotator principle. Perikaryal loss was progressive during the early study period but stabilized 45 days after nerve injury. The mean number (n) of all neurons in intact L5 DRG was 16,400 (S.D. = 2,000). The loss of perikarya was 16% (P < 0.05) after four days, 15% (P < 0.05) after 15 days, 30% (P = 0.059) after 45 days, and 34% (P < 0.05) after 120 days. B cells were lost at an earlier time than were A cells, and the B cell loss was more pronounced (39% vs. 22%, respectively, after 120 days). 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