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Thermal inactivation and molecular forms of the estrogen receptor: Effects of molybdate

1981; Elsevier BV; Volume: 103; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(81)90481-2

1090-2104

Irène Joab, Gérard Redeuilh, C Secco, Christine Radanyi, Étienne-Émile Baulieu, Hélène Richard‐Foy,

Coagulation, Bradykinin, Polyphosphates, and Angioedema

Exposure at 40°C of low salt calf uterine cytosol leads to the “transformation” of the estradiol-receptor complexes in 5 min, immediately followed by the formation of thermostable > 12 S “aggregated” receptor forms. Molybdate prevents this phenomenon but does not reverse it. Molybdate has a protective effect against thermal inactivation of the 9 S non-aggregated form of the receptor in the absence of hormone. In the presence of molybdate, the inactivation rate of this 9 S receptor is the same with and without hormone, and follows a first order reaction (t12 = 7–8 min). The biphasic kinetics of thermo-inactivation of estradiol-receptor complexes is ascribable to the relative amounts of non-aggregated and aggregated forms.