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Histochemical Observations on Carbohydrates in Connective Tissue Structures and Basement Membranes of Hemi‐ and Cephalochordates

1984; Wiley; Volume: 65; Issue: 2 Linguagem: Inglês

10.1111/j.1463-6395.1984.tb00815.x

0001-7272

Ulrich Welsch, Udo Schumacher,

Vector-borne infectious diseases

Abstract Connective tissue components and light microscopical basement membranes of Saccoglossus horsti (Enteropneusta, Hemichordata) and Branchiostoma lanceolatum have been studied with Aldehyde Fuchsin, the PAS‐reaction, Alcian Blue (pH 0.2) and fluorescein conjugated (FITC) lectins: concanavalin A (Con A), wheat germ agglutinin (WGA), soy bean agglutinin (SBA), leucoagglutinin (LA), Griffonia (Bandeiraea) simplicifolia agglutinin (GSA I), Griffonia (Bandeiraea) simplicifolia isolectin B 4 (GS I B4). In Saccoglossus and Branchiostoma , both the PAS‐reaction and Alcian blue give a good general survey over the distribution of the principal basement membranes and connective tissue structures. Lectin binding proved less intensive in Saccoglossus than in Branchiostoma , in which FITC‐Con A, FITC‐GSA and FITC‐WGA react strongly with the dermal (especially in the metapleural folds) and axial connective tissue, as well as the myosepta, the gill arch skeleton and numerous basement membranes. Con A outlines distinctly the major blood vessels in the pharyngeal area. Con A, WGA, GSA and GSI B 4 are markers for basement membranes.