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Direct DNA isolation from solid biological sources without pretreatments with proteinase-K and/or homogenization through automated DNA extraction

2007; Elsevier BV; Volume: 103; Issue: 3 Linguagem: Inglês

10.1263/jbb.103.242

1389-1723

Jang‐Seu Ki, Ki Byum Chang, Hee June Roh, Bong Youb Lee, Joon Yong Yoon, Gi Young Jang,

Bacteriophages and microbial interactions

Genomic DNA from solid biomaterials was directly isolated with an automated DNA extractor, which was based on magnetic bead technology with a bore-mediated grinding (BMG) system. The movement of the bore broke down the solid biomaterials, mixed crude lysates thoroughly with reagents to isolate the DNA, and carried the beads to the next step. The BMG system was suitable for the mechanical homogenization of the solid biomaterials and valid as an automated system for purifying the DNA from the solid biomaterials without the need for pretreatment or disruption procedures prior to the application of the solid biomaterials.