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Chondrocyte Cultivation in Hyaluronan-Tyramine Cross-Linked Hydrogel

2015; Taylor & Francis; Volume: 64; Issue: 13 Linguagem: Inglês

10.1080/00914037.2014.996715

1563-535X

Lukáš Kučera, Radka Weinfurterová, Jana Dvořáková, Jan Kučera, Martin Pravda, Marcela Foglarová, Karol Švík, Pavel Klein, Vladimı́r Velebný, Lukáš Kubala,

Silk-based biomaterials and applications

The application of chondrocyte-seeded hydrogels, such as tyramine-substituted hyaluronan, is a highly promising strategy for damaged cartilage treatments. The rheological characteristics of hyaluronan-tyramine derivative scaffold were determined together with the hydrogel swelling, the water uptake, and the morphological characterization. The chondrocytes seeded in hyaluronan-tyramine hydrogel were viable and expressed markers typical for differentiated chondrocytes with no signs of hypertrophy or extensive expression of matrix metalloproteinases. The lyophilized unseeded hyaluronan-tyramine scaffolds were also implantated into trochlea osteochondral defects in rabbits. In conclusion, the hyaluronan-tyramine derivative was proven to be advisable material as a scaffold for cartilage reconstitution.