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Cardia bifida in chick embryos: Anterior and posterior defects produced by transplanting tritiated thymidine‐labeled grafts medial to the heart‐forming regions

1970; Wiley; Volume: 3; Issue: 2 Linguagem: Inglês

10.1002/tera.1420030205

2472-1727

Glenn C. Rosenquist,

Congenital Heart Disease Studies

Abstract Anterior and posterior cardia bifida were produced in chick embryos by transplanting tritiated thymidine‐labeled grafts into host embryos, anterior to the anterior end of the streak and medial to the heart‐forming mesoderm, i.e., into areas destined for ventral gut or yolk sac. Anterior cardia bifida resulted when the nonintegrated part of the graft prevented the splanchnic folds from meeting at the midline to form the anterior end of the heart; this anomaly always appeared without corresponding anomalies in the endoderm and ectoderm layers. Posterior cardia bifida resulted when the part of the graft that had not been integrated into the host tissue interfered with the formation of the foregut. The position of the labeled cells in the endocardium, myocardium, mesocardium, endoderm, and yolk sac was determined by radioautography. Although cardia bifida has no known counterparts in the pathological anatomy of human congenital heart disease, study of the condition gives significant insight into the relations of the primary germ layers.