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Genome-wide microRNA profiling in human fetal nervous tissues by oligonucleotide microarray

2006; Springer Science+Business Media; Volume: 22; Issue: 11 Linguagem: Inglês

10.1007/s00381-006-0173-9

1433-0350

Jianjun Zhao, You-Jia Hua, Da-Guang Sun, Xian-Xin Meng, Hua-Sheng Xiao, Xu Ma,

Circular RNAs in diseases

Our objective was to develop an oligonucleotide DNA microarray (OMA) for genome-wide microRNA profiling and use this method to find miRNAs, which control organic development especially for nervous system. Eighteen organic samples included cerebrum and spinal cord samples from two aborted human fetuses. One was 12 gestational weeks old (G12w) and the other was 24 gestational weeks old (G24w). Global miRNA expression patterns of different organs were investigated using OMA and Northern blot. The OMA revealed that 72–83% of miRNAs were expressed in human fetal organs. A series of microRNAs were found specifically and higher-expressed in the human fetal nervous system and confirmed consistently by Northern blot, which may play a critical role in nervous system development.