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Automated assay of creatinine in serum as simplified by the use of immobilized enzymes, creatinine deiminase, and glutamate dehydrogenase

1983; Elsevier BV; Volume: 134; Issue: 1 Linguagem: Inglês

10.1016/0003-2697(83)90261-0

1096-0309

Masayoshi Tabata, Takahiro Kido, Masayuki Totani, Takashi Murachi,

Metabolomics and Mass Spectrometry Studies

A method for the automated analysis of creatinine in serum using immobilized enzymes in column form which does not require a blank correction and can be run in a single buffer of pH 7.5 is described. The method was based on the determination of ammonia formed by the action of creatinine deiminase. Endogenous ammonia in serum was removed by an immobilized glutamate dehydrogenase column prior to the action of creatinine deiminase also immobilized and used in column form. The present method was found to give perfect linearity of the data up to 0.10 g creatinine/liter with satisfactory precision, reproducibility, and accurate reaction recoveries. The immobilized enzyme reactor unit showed good operational stability for a 2-month period, during which time it was repeatedly used for analyses over 2000 times. The results correlated satisfactorily with those obtained by other well-established methods.