Simple step gradient elution of the major high-energy compounds and their catabolites in cardiac muscle using high-performance liquid chromatography
1986; Elsevier BV; Volume: 377; Linguagem: Inglês
10.1016/s0378-4347(00)80771-9
ISSN1872-812X
AutoresEmily A. Hull‐Ryde, William R. Lewis, Charles D. Veronee, James E. Lowe,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoThe majority of high-energy nucleotides and their catabolites are separated in a single 13-min run using reversed-phase high-performance liquid chromatography. Economical step gradient elution of these compounds with a Nova Pak-A C18, 5-μm, 10 cm X 8 mm column accomplishes this separation with recoveries of 94–100% and sensitivities of 1–5 pmol. Furthermore, the total adenine nucleotide pool can now be quantitated precisely in a simple and easily automated procedure.
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