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Detection of insertion/deletion polymorphisms from challenged samples using the Investigator DIPplex ® Kit

2014; Elsevier BV; Volume: 16; Linguagem: Inglês

10.1016/j.fsigen.2014.11.020

1878-0326

Rebecca C. Klein, Cédric Neumann, Reena Roy,

Identification and Quantification in Food

This research focuses on detection of bi-allelic insertion/deletion polymorphisms (InDels) from challenged samples using the Investigator DIPplex® Kit from Qiagen. The study included analyzing body fluids from humans, as well as pristine and degraded samples. For the purpose of assessing species specificity, samples from various animals were included. At first, an analytical threshold (AT) for the detection of alleles was established based on an assessment of the noise in the system. Then, InDel profiles were obtained from samples exposed to detrimental environmental conditions, washed bloodstains, lipsticks, ChapStick®, ancient Croatian bone samples, and every day products such as toothbrushes and dental floss. Concordant profiles were obtained from different body fluids of the same donor. InDel profiles were also generated successfully when body fluids were deposited on substrates and directly amplified without pre-treatment with buffer or washing reagents. InDels can provide additional information when only partial STR profiles are generated from challenged samples.