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Determination of unsaturated fatty acid composition by high‐resolution nuclear magnetic resonance spectroscopy

1998; Wiley; Volume: 75; Issue: 12 Linguagem: Inglês

10.1007/s11746-998-0295-1

1558-9331

Yuko Miyake, Kazuhisa Yokomizo, Narihide Matsuzaki,

Analytical Chemistry and Chromatography

Abstract High‐resolution nuclear magnetic resonance (NMR) spectroscopy provides useful data for analyzing fatty acid compositions of edible vegetable oils. Quantitation of each fatty acid was carried out by evaluation of particular peaks. According to the 1 H NMR method, terminal methyl protons, divinyl protons, and allyl protons are useful to calculate linolenic acid, linoleic acid, and oleic acid, respectively. The ω‐2 carbon, divinyl carbon, and allylic carbons were used for calculation of these acids by the 13 C NMR method. Compositional results obtained by NMR coincided well with those of the conventional gas chromatography (GC) method. Results from 13 C NMR were in better agreement with those from GC than were the results obtained by the 1 H NMR method.