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Evaluation of a New Prestorage Leukoreduction Filter for Red Blood Cell Units

1997; Wiley; Volume: 72; Issue: 2 Linguagem: Inglês

10.1046/j.1423-0410.1997.7220101.x

1423-0410

James P. AuBuchon, M. Dean Elfath, Mark A. Popovsky, Robert R. Stromberg, Constance Pickard, Louise Herschel, Pamela Whitley, Deanna McNeil, Nancy L. Arnold, James L. O'Connor,

Blood disorders and treatments

Abstract Background and objectives: Prestorage leukoreduction offers a variety of potential benefits and is becoming more commonly practiced. The LeukoNet™ prestorage leukoreduction filtration system is intended for leukoreduction of red blood cells and uses a vent to allow automatic drainage of red cells from the filter. Materials and methods: We studied the functional characteristics and the in‐vivo and in‐vitro properties of leukoreduced AS‐1 Red Blood Cells prepared with this new system. Units of AS‐1 Red Blood Cells were filtered at 4°C through the LeukoNet™ filter 24–48 h after collection and stored under usual conditions for 42 days. Residual leukocytes were enumerated using a Nageotte chamber or with a polymerase chain reaction (PCR) technique. In the clinical trial (phase one), 21 donors had units stored with and without leukoreduction for 42 days; biochemical assays were done before and after storage, and 51 Cr/ 99 mTc red cell recovery studies at the end of the storage period. Results: Leukocyte content after filtration was 3.2±2.6×10 4 /unit (n = 21), and all units had <1×10 5 leukocytes (median: 3.8×10 4 ). In‐vivo paired studies showed no difference in 24‐hour recovery (control: 82.1±5.8%; test: 82.9±6.0%). Hemolysis was halved with leukoreduction (0.59±0.30 vs. 0.29±0.11%; p<0.05), and glucose consumption was reduced by 5% compared to control units (p = <0.05). Other biochemical parameters showed no differences. In the practical trial (phase two), filtration time was 41±23 min. With a residual leukocyte content of 6.6±4.9×10 4 /unit and 14±3% red cell loss (n = 84). Six additional units underwent leukocyte enumeration by PCR and had 2.6±1.1×10 4 residual leukocytes. Conclusions: Under the conditions studied, the LeukoNet™ leukoreduction filtration system produces about 4–5 log 10 leukocyte content reduction.