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Production of raw cassava starch-digestive glucoamylase by Rhizopus sp. in liquid culture

1988; Elsevier BV; Volume: 66; Issue: 4 Linguagem: Inglês

10.1016/0385-6380(88)90005-2

1878-2108

Hiroshi Nishise, Akira Fuji, Makoto Ueno, Vitchuporn Vongsuvanlert, Yoshiki Tani,

Cassava research and cyanide

Among about 200 Rhizopus strains isolated in Thailand, Rhizopus sp. MB46 was selected as a producer of raw cassava starch-digestive glucoamylase. Rice bran was effective for the enzyme production in a solid culture as well as wheat bran. Addition of turpentine oil into the rice bran solid culture increased the productivity. Rhizopus sp. MB46 was found to produce glucoamylase in a liquid culture containing 1% rice bran but not in one consisting of 10% raw cassava starch of 2% glucose. The productivity per 1 g solids in the medium in liquid culture was finally improved 6-times by utilization of n-hexane-treated rice bran, supplement of 0.1% meat extract and addition of gauze as a support. The activity was superior to that in turpentine oil-supplemented solid culture.