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Expression of the p80 region of bovine viral diarrhea virus and identification of specific antibodies to this recombinant protein in bovine sera

1991; Elsevier BV; Volume: 178; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(91)91039-f

1090-2104

Jimmy Kwang, Steven R. Bolin, E. Travis Littledike, Edward J. Dubovi, Rubén O. Donis,

Viral gastroenteritis research and epidemiology

A 917-base pair segment of the bovine viral diarrhea virus (BVDV) genome encoding part of the p80 region was cloned into plasmid Gex-2T expression vector for expression as a fusion protein with glutathione-S-transferase (GST). When the p80 and GST sequences were in the same reading frames, the resulting GST-p80 fusion protein had a molecular mass of 58 kilodaltons (kDa) in SDS-PAGE. Extracts of control E.coli carrying only the vector plasmid (Gex-2T) did not contain this new 58-kDa protein band. Mouse monoclonal antibody specific to BVDV-p80 recognized this recombinant protein. Seventy cattle sera that had an SN titer (to TGAC isolate of cytopathic BVDV) greater than 1:8 reacted with this recombinant protein in Western blots. Of 28 cattle sera that had SN titers ≤ 1:8, only one serum tested positive on Western blots.