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Non‐selective afferent innervation develops in embryonic mouse spinal cord‐dorsal root ganglia explants chronically exposed to GM1 ganglioside

1989; Wiley; Volume: 7; Issue: 1 Linguagem: Inglês

10.1016/0736-5748(89)90047-6

1873-474X

Robert E. Baker, Daan Gaasbeek Janzen,

Nerve injury and regeneration

Projection patterns of dorsal root ganglion afferents within organotypic explants of fetal mouse spinal cord were examined histologically using a horseradish peroxidase staining method. All explants were also examined electrophysiologically and showed spontaneous and evoked bioelectric discharges, which were evenly distributed across the cord explants. Explants chronically grown in a serum-free medium containing the monosialoganglioside GM1 failed to develop dorsal cord selectivity as was previously observed when a mixture of gangliosides was used. It therefore appears that not all gangliosides can induce selectivity in this model.