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EFFECTS OF ALPROSTADIL AND PRAZOSIN ON MOTILITY, VIABILITY AND MEMBRANE INTEGRITY OF HUMAN SPERM

1998; Lippincott Williams & Wilkins; Volume: 159; Issue: 5 Linguagem: Inglês

10.1097/00005392-199805000-00041

1527-3792

Wayne J.G. Hellstrom, Run Wang, Craig A. Peterson, John Varady, Neil Gesundheit, Suresh C. Sikka,

Sperm and Testicular Function

No AccessJournal of UrologyClinical Urology: Original Articles1 May 1998EFFECTS OF ALPROSTADIL AND PRAZOSIN ON MOTILITY, VIABILITY AND MEMBRANE INTEGRITY OF HUMAN SPERM WAYNE J.G. HELLSTROM, RUN WANG, CRAIG A. PETERSON, JOHN C. VARADY, NEIL GESUNDHEIT, and SURESH C. SIKKA WAYNE J.G. HELLSTROMWAYNE J.G. HELLSTROM More articles by this author , RUN WANGRUN WANG More articles by this author , CRAIG A. PETERSONCRAIG A. PETERSON More articles by this author , JOHN C. VARADYJOHN C. VARADY More articles by this author , NEIL GESUNDHEITNEIL GESUNDHEIT More articles by this author , and SURESH C. SIKKASURESH C. SIKKA More articles by this author View All Author Informationhttps://doi.org/10.1097/00005392-199805000-00041AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We evaluated the effects of alprostadil, prazosin hydrochloride, and alprostadil/prazosin hydrochloride, agents used in the clinical treatment of male erectile dysfunction, on the motility, viability and membrane integrity of human sperm. Materials and Methods: Ten healthy volunteers provided semen samples that were incubated with 0.4 mg./ml. alprostadil, 0.1 and 0.2 mg./ml. prazosin hydrochloride and 0.4 mg./ml. alprostadil plus 0.1 mg./ml. prazosin hydrochloride for 2 hours. Control incubations included polyethylene glycol 1450, the formulation vehicle for the clinical use of alprostadil and prazosin, and Ham's F-10 buffer. Serial evaluations of percent sperm motility, percent viability, membrane function (by hypo-osmotic swelling test) and several computer generated measurements of sperm motion, including straight line velocity, curvilinear velocity, linearity and amplitude of lateral head displacement, were made. Results: None of the agents had a significant impact on the percentage of motile or viable sperm or on sperm membrane function. Incubation with 0.2 mg./ml. prazosin reduced straight line velocity and curvilinear velocity significantly compared with the other agents. These changes were most likely a direct result of the viscosity of the 0.2 mg./ml. prazosin solution and not a cellular or metabolic effect on the sperm. Conclusions: Alprostadil and prazosin hydrochloride at doses used in transurethral therapy for erectile dysfunction have no effect on the motility, viability and membrane integrity of human sperm. 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Google Scholar 15 : Platelet activating factor and pentoxifylline as human sperm cryoprotectants. Fertil. Steril.1993; 60: 711. Google Scholar From the Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana, and VIVUS Inc., Menlo Park, California.© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 159Issue 5May 1998Page: 1559-1562 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information WAYNE J.G. HELLSTROM More articles by this author RUN WANG More articles by this author CRAIG A. PETERSON More articles by this author JOHN C. VARADY More articles by this author NEIL GESUNDHEIT More articles by this author SURESH C. SIKKA More articles by this author Expand All Advertisement PDF downloadLoading ...