Cell clones contrasted in growth, morphology and pigmentation isolated from a callus culture of Atropa belladonna var. Lutea
1971; Elsevier BV; Volume: 10; Issue: 11 Linguagem: Inglês
10.1016/s0031-9422(00)97246-7
ISSN1873-3700
AutoresM. R. Davey, Michael Fowler, H. E. Street,
Tópico(s)Plant Stress Responses and Tolerance
ResumoThree cell clones differing from one another in growth rate and growth pattern in suspension culture, in nutrient requirement, in callus and cell morphology, in cellular fine structure and in pigmentation have been isolated from a callus of Atropa belladonna var. lutea Döll of seedling root origin. Two of the cell clones (HC and FC) became green when cultured in light and developed a higher content of chloroplast pigments than the original stock callus, whereas the third clone (W) was of very low pigment content and developed only amyloplasts. The structure of the chloroplasts and the contents of individual chloroplast pigments in the green clones are compared with those of leaf cells of the species. Preliminary work on the photosynthetic activity of the green clone HC is reported. The influence of sucrose supply and of the level and kind of auxin (NAA or 2,4-D) on growth and pigmentation of the green clones is described. The sequential changes in plastid structure and in chloroplast pigment contents which occur when dark-grown clones HC and FC are transferred to light or returned to darkness after growth in light have been followed.
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