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Multipotent cells can be generated in vitro from several adult human organs (heart, liver, and bone marrow)

2007; Elsevier BV; Volume: 110; Issue: 9 Linguagem: Inglês

10.1182/blood-2006-11-055566

1528-0020

Antonio Paolo Beltrami, Daniela Cesselli, Natascha Bergamin, Patrizia Marcon, Silvia Rigo, Elisa Puppato, Federica DˈAurizio, Roberto Verardo, Silvano Piazza, Angela Pignatelli, Alessandra Poz, Umberto Baccarani, Daniela Damiani, Renato Fanin, Laura Mariuzzi, Nicoletta Finato, Paola Masolini, Silvia Burelli, Ottorino Belluzzi, Claudio Schneider, Carlo Alberto Beltrami,

Renal and related cancers

Abstract The aims of our study were to verify whether it was possible to generate in vitro, from different adult human tissues, a population of cells that behaved, in culture, as multipotent stem cells and if these latter shared common properties. To this purpose, we grew and cloned finite cell lines obtained from adult human liver, heart, and bone marrow and named them human multipotent adult stem cells (hMASCs). Cloned hMASCs, obtained from the 3 different tissues, expressed the pluripotent state–specific transcription factors Oct-4, NANOG, and REX1, displayed telomerase activity, and exhibited a wide range of differentiation potential, as shown both at a morphologic and functional level. hMASCs maintained a human diploid DNA content, and shared a common gene expression signature, compared with several somatic cell lines and irrespectively of the tissue of isolation. In particular, the pathways regulating stem cell self-renewal/maintenance, such as Wnt, Hedgehog, and Notch, were transcriptionally active. Our findings demonstrate that we have optimized an in vitro protocol to generate and expand cells from multiple organs that could be induced to acquire morphologic and functional features of mature cells even embryologically not related to the tissue of origin.