Genome sequencing reveals agronomically important loci in rice using MutMap
2012; Nature Portfolio; Volume: 30; Issue: 2 Linguagem: Inglês
10.1038/nbt.2095
ISSN1546-1696
AutoresAkira Abe, Shunichi Kosugi, Kentaro Yoshida, Satoshi Natsume, Hiroki Takagi, Hiroyuki Kanzaki, Hideo Matsumura, Kakoto Yoshida, Chikako Mitsuoka, Muluneh Tamiru, Hideki Innan, Liliana M. Cano, Sophien Kamoun, Ryohei Terauchi,
Tópico(s)Genetic Mapping and Diversity in Plants and Animals
ResumoGenetic analysis of agronomic traits in crops is complicated by the long generation times and challenges of growing and phenotyping plants in large field trials. Abe et al. show how whole genome resequencing can be used to identify the genetic basis of subtle phenotypic traits in rice. The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type line and then selfed, allowing unequivocal segregation in second filial generation (F2) progeny of subtle phenotypic differences. This approach is particularly amenable to crop species because it minimizes the number of genetic crosses (n = 1 or 0) and mutant F2 progeny that are required. We applied MutMap to seven mutants of a Japanese elite rice cultivar and identified the unique genomic positions most probable to harbor mutations causing pale green leaves and semidwarfism, an agronomically relevant trait. These results show that MutMap can accelerate the genetic improvement of rice and other crop plants.
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