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An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli

2008; Volume: 4; Issue: 4 Linguagem: Inglês

10.3844/ajbbsp.2008.422.424

1553-3468

Younes Ghasemi, Alireza Ebrahimine, S. Rasoul-Ami, Gholamreza Zarrini, Mohammad Bagher Ghoshoon, Mohammad Javad Raee, Mohammad Hossein Morowvat, Farshid Kafilzadeh, Aboozar Kazemi,

Biochemical Acid Research Studies

Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.