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ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna

2007; UNIVERSIDADE FEDERAL DE MINAS GERAIS; Volume: 59; Issue: 5 Linguagem: Inglês

10.1590/s0102-09352007000500040

1678-4162

Darleise de Souza Oliveira, Áurea Valadares Folgueras-Flatschart, R.B. Flatschart, J.S. Resende, J.T. Abreu, Nélson Rodrigo da Silva Martins,

T-cell and Retrovirus Studies

An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation.