Ultrasensitive electrochemical detection of cytokeratin-7, using Au nanowires based biosensor
2007; Elsevier BV; Volume: 129; Issue: 2 Linguagem: Inglês
10.1016/j.snb.2007.09.080
ISSN1873-3077
AutoresSheetal Patil, Aleksandra Zajac, Tatyana Zhukov, Shekhar Bhansali,
Tópico(s)Molecular Junctions and Nanostructures
ResumoCytokeratin-7 (CK-7), a protein expressed in epithelial tissue, used to differentiate between types of cancers has been detected successfully by employing gold (Au) nanowires as a template for the enzyme immunoassay. Functionalized Au nanowires were used to enhance the sensitivity and selectivity of the cancer biomarkers detection. An enzymatic reaction between alkaline phosphatase enzymes with the p-nitrophenyl phosphate substrate resulted in an electroactive p-nitrophenol and redox active intermediate hydroquinone that has been detected electrochemically. A strong dependence of the anodic peak current with the concentrations of CK-7 resulted in detection down to 10 ng/mL concentration. Additionally, the cross-validation was assured using quantum dots-655 fluorescent markers.
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