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Speeding Up Three-Dimensional Protein NMR Experiments to a Few Minutes

2006; American Chemical Society; Volume: 128; Issue: 28 Linguagem: Inglês

10.1021/ja062025p

1943-2984

Paul Schanda, Hélène Van Melckebeke, Bernhard Brutscher,

Advanced NMR Techniques and Applications

We demonstrate for different protein samples that three-dimensional HNCO and HNCA correlation spectra may be recorded in a few minutes acquisition time using the band-selective excitation short-transient sequences presented here. This opens new perspectives for the NMR structural investigation of unstable protein samples and real-time site-resolved studies of protein kinetics.