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Application of a qualitative and quantitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products

2012; Elsevier BV; Volume: 136; Issue: 2 Linguagem: Inglês

10.1016/j.foodchem.2012.08.088

1873-7072

Kôsuke Nakamura, Hiroshi Akiyama, Yuki Takahashi, Tomoko Kobayashi, Akio Noguchi, Kiyomi Ohmori, Masaki Kasahara, Kazumi Kitta, Hiroyuki Nakazawa, Kazunari Kondo, Reiko Teshima,

Insect Resistance and Genetics

Genetically modified (GM) papaya (Carica papaya L.) line 55-1 (55-1), which is resistant to papaya ringspot virus infection, has been marketed internationally. Many countries have mandatory labeling regulations for GM foods, and there is a need for specific methods for detecting 55-1. Here, an event- and construct-specific real-time polymerase chain reaction (PCR) method was developed for detecting 55-1 in papaya products. Quantitative detection was possible for fresh papaya fruit up to dilutions of 0.001% and 0.01% (weight per weight [w/w]) for homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The limit of detection and quantification was as low as 250 copies of the haploid genome according to a standard reference plasmid. The method was applicable to qualitative detection of 55-1 in eight types of processed products (canned papaya, pickled papaya, dried fruit, papaya-leaf tea, jam, puree, juice, and frozen dessert) containing papaya as a main ingredient.