Structural investigation of the capsular polysaccharide from Klebsiella serotype K-34 and its characterization by N.M.R. spectroscopy
1982; Elsevier BV; Volume: 101; Issue: 2 Linguagem: Inglês
10.1016/s0008-6215(00)80998-2
ISSN1873-426X
AutoresJ.P. Joseleau, Francis Michon, Michel R. Vignon,
Tópico(s)Amino Acid Enzymes and Metabolism
ResumoAbstract The repeating unit of the capsular polysaccharide from Klebsiella type K-34 has been established by methylation, partial hydrolysis, and Smith degradation to consist of a hexasaccharide repeating-unit built up of four l -rhamnose, one d -glucose, and one d -galacturonic acid residues. The anomeric configurations of the linkages was determined by proton and 13C-n.m.r. spectroscopy at each step of the degradation procedures. Further evidence for the configurations of the glycosidic linkages involved the use of proton T1 relaxation-times and oxidation by chromium trioxide. The data allowed assignment of the following structure for the repeating unit:
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