iPS Cells Can Support Full-Term Development of Tetraploid Blastocyst-Complemented Embryos
2009; Elsevier BV; Volume: 5; Issue: 2 Linguagem: Inglês
10.1016/j.stem.2009.07.001
ISSN1934-5909
AutoresLan Kang, Jianle Wang, Yu Zhang, Zhaohui Kou, Shaorong Gao,
Tópico(s)Renal and related cancers
ResumoTo our knowledge, for the first time, we demonstrate that induced pluripotent stem cells (iPSCs) can autonomously generate full-term mice via tetraploid blastocysts complementation. Differentiated somatic cells can be reprogrammed into iPSCs by forced expression of four transcription factors—Oct4, Sox2, Klf4, and c-Myc. However, it has been unclear whether reprogrammed iPSCs are fully pluripotent, resembling normal embryonic stem cells (ESCs), as no iPSC lines have shown the ability to autonomously generate full-term mice after injection into tetraploid blastocysts. Here we provide evidence demonstrating that an iPSC line induced by the four transcription factors can be used to generate full-term mice from complemented tetraploid blastocysts and thus appears to be fully pluripotent. This work serves as a proof of principle that iPSCs can in fact generate full-term embryos by tetraploid complementation.
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