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Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa

2000; Wiley; Volume: 58; Issue: 1 Linguagem: Inglês

10.1002/1098-2795(200101)58

1098-2795

Moira K. O’Bryan, Kimberly Sebire, Andreas Meinhardt, Kimberly Edgar, Hong-Hooi Keah, Milton T. W. Hearn, David M. de Kretser,

Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

Molecular Reproduction and DevelopmentVolume 58, Issue 1 p. 116-125 Gamete Biology Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa Moira K. O'Bryan, Corresponding Author Moira K. O'Bryan [email protected] Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaMoira K. O'Bryan, Ph.D., Monash Institute of Reproduction and Development, Monash Medical Centre, 246 Clayton Road, Clayton, 3168. Australia.Search for more papers by this authorKimberly Sebire, Kimberly Sebire Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaSearch for more papers by this authorAndreas Meinhardt, Andreas Meinhardt The Department of Anatomy and Cell Biology, Philipps-University of Marburg, Marburg, GermanySearch for more papers by this authorKimberly Edgar, Kimberly Edgar Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaSearch for more papers by this authorHong-Hooi Keah, Hong-Hooi Keah The Centre for Bioprocess Technology, Department of Biochemistry and Molecular Biology, Monash University, Clayton, AustraliaSearch for more papers by this authorMilton T.W. Hearn, Milton T.W. Hearn The Centre for Bioprocess Technology, Department of Biochemistry and Molecular Biology, Monash University, Clayton, AustraliaSearch for more papers by this authorDavid M. de Kretser, David M. de Kretser Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaSearch for more papers by this author Moira K. O'Bryan, Corresponding Author Moira K. O'Bryan [email protected] Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaMoira K. O'Bryan, Ph.D., Monash Institute of Reproduction and Development, Monash Medical Centre, 246 Clayton Road, Clayton, 3168. Australia.Search for more papers by this authorKimberly Sebire, Kimberly Sebire Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaSearch for more papers by this authorAndreas Meinhardt, Andreas Meinhardt The Department of Anatomy and Cell Biology, Philipps-University of Marburg, Marburg, GermanySearch for more papers by this authorKimberly Edgar, Kimberly Edgar Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaSearch for more papers by this authorHong-Hooi Keah, Hong-Hooi Keah The Centre for Bioprocess Technology, Department of Biochemistry and Molecular Biology, Monash University, Clayton, AustraliaSearch for more papers by this authorMilton T.W. Hearn, Milton T.W. Hearn The Centre for Bioprocess Technology, Department of Biochemistry and Molecular Biology, Monash University, Clayton, AustraliaSearch for more papers by this authorDavid M. de Kretser, David M. de Kretser Monash Institute of Reproduction and Development, Monash Medical Centre, Clayton, AustraliaSearch for more papers by this author First published: 04 December 2000 https://doi.org/10.1002/1098-2795(200101)58:1 3.0.CO;2-8Citations: 66AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Previously we reported the cloning of a member of the cysteine-rich secretory protein family, tpx-1, from a testis expression library using an outer dense fiber (ODF)-specific antiserum. Using immunohistochemical and immunoelectron microscopic techniques and Western blotting of purified sperm tail components, we have determined that tpx-1 exists as 25 and 27 kDa proteins in two components of rat spermatid: the ODFs and the acrosome. Tpx-1 mRNA is first expressed in the late pachytene spermatocytes, but the production of these tpx-1 proteins is translationally delayed for 4–5 days before being incorporated into the developing sperm acrosome, surrounding the elongating and condensing spermatid nucleus. Concurrent with sperm head formation, tpx-1 protein was incorporated into the developing sperm tail, and specifically the ODFs. The tpx-1 protein was seen within structures resembling granulated bodies in the cytoplasmic lobe of elongating spermatids and was incorporated subsequently into the growing tail in a manner consistent with ODF development. In addition, tpx-1 protein was localized at the ultrastructural level of the connecting piece of the neck and longitudinal columns of the fibrous sheath, suggesting common protein components in these cytoskeletal structures. As such, tpx-1 may have functional significance in the processes of sperm head development and tail function. Mol. Reprod. Dev. 58:116–125, 2001. © 2001 Wiley-Liss, Inc. REFERENCES Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215: 403–410. 10.1016/S0022-2836(05)80360-2 CASPubMedWeb of Science®Google Scholar Baltz JM, Williams PO, Cone RA. 1990. Dense fibres protect mammalian sperm against damage. 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