Novel extracellular enzymes (ligninases) of Phanerochaete chrysosporium
1985; Oxford University Press; Volume: 28; Issue: 1 Linguagem: Inglês
10.1111/j.1574-6968.1985.tb00776.x
ISSN1574-6968
AutoresVan-Ba Huynh, Ronald L. Crawford,
Tópico(s)Lignin and Wood Chemistry
Resumo3 New spectrophotometric enzyme assays were developed for the study of microbial lignin-degrading enzymes. The conversion of 2-methoxy-3-phenylbenzoic acid to 2-hydroxy-3-phenylbenzoic acid led to the discovery of an extracellular, aromatic methyl ether demethylase produced by the white-rot fungus Phanerochaete chrysosporium. The conversion of methyl 2-hydroxy-3-phenylbenzoate to 2-hydroxy-3-phenylbenzoic acid allowed the identification of an extracellular, aromatic methyl ester esterase produced by this fungus. The Phanerochaete sp. also excreted an enzyme complex that oxidized 4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one, probably to aliphatic products. All 3 novel enzyme activities were produced together with, and probably comprise a part of, the Phanerochaete ligninolytic enzyme complex. Unlike previously known ligninases, these enzymes did not oxidize 3,4-dimethoxybenzyl alcohol. All 3 were H2O2-dependent and were activated by Mn2+ ions.
Referência(s)