Thermitase – eine thermostabile Serinprotease. I. Synthese von Alaninpeptidestern als Substrate des Enzyms
1984; Wiley; Volume: 326; Issue: 1 Linguagem: Inglês
10.1002/prac.19843260106
ISSN0021-8383
Autores Tópico(s)Protein Hydrolysis and Bioactive Peptides
ResumoThermitase – A Thermostable Serine Protease. I. Synthesis of Alanine Peptide Esters as Substrates of the Enzyme The synthesis of N‐acetylated and N‐succinylated peptide esters of alanine is described. The peptides are built up from the Z‐ Abkürzung: Z = Benzyloxycarbonyl, Ac = Acetyl, Suc = Succinyl, OMe = Methylester, MA = Mischanhydridmethode, E = Ether, PE = Petrolether, Etac = Ethylacetat, AcOH = Essigsäure, Dibu = n‐Dibutylether, DMSO = Dimethylsulfoxid, DMF = Dimethylformamid, TEA = Triethylamin, Ac 2 O = Essigsäureanhydrid protected peptides by liquid phase fragment condensation and by acylation of the deprotected peptide esters. The kinetic parameters K m and K cat of some compounds are presented.
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