Viral Nanoparticles Donning a Paramagnetic Coat: Conjugation of MRI Contrast Agents to the MS2 Capsid
2006; American Chemical Society; Volume: 6; Issue: 6 Linguagem: Inglês
10.1021/nl060378g
ISSN1530-6992
AutoresElizabeth A. Anderson, Steven Isaacman, David S. Peabody, Edwin Y. Wang, James W. Canary, Kent Kirshenbaum,
Tópico(s)Protein purification and stability
ResumoA nanoparticle magnetic resonance imaging (MRI) contrast agent was developed by conjugation of more than 500 gadolinium chelate groups onto a viral capsid. The high density of paramagnetic centers and slow tumbling rate of modified MS2 capsids provided enhanced T1 relaxivities up to 7200 mM-1s-1 per particle. A bimodal imaging agent was generated by sequential conjugation of fluorescein and Gd3+ chelate. These results illustrate the potential for engineering natural protein assemblies to address bionanotechnology applications.
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