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Nanomaterial-based surface-assisted laser desorption/ionization mass spectrometry of peptides and proteins

2010; American Chemical Society; Volume: 21; Issue: 7 Linguagem: Inglês

10.1016/j.jasms.2010.02.028

1879-1123

Cheng-Kang Chiang, M.F. Chiang, Zong‐Hong Lin, Guo-Yu Lan, Yang‐Wei Lin, Huan‐Tsung Chang,

Advanced biosensing and bioanalysis techniques

We have investigated six nanomaterials for their applicability as surfaces for the analyses of peptides and proteins using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Gold nanoparticles (NPs) were useful nanomateriais for small analytes (e.g., glutathione); Pt nanosponges and Fe3O4 NPs were efficient nanomaterials for proteins, with an upper detectable mass limit of ca. 25 kDa. Nanomateriais have several advantages over organic matrices, including lower limits of detection for small analytes and lower batch-to-batch variations (fewer problems associated with "sweet spois"), when used in laser desorption/ionization mass spectrometry.