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Continuous-flow fast atom bombardment-mass spectrometry of permethylated oligosaccharides: A comparative study of direct mixture analysis with packed capillary column liquid chromatography-fast atom bombardment-mass spectrometry

1988; Elsevier BV; Volume: 168; Issue: 1 Linguagem: Inglês

10.1016/0003-2697(88)90024-3

1096-0309

Patrick Boulenguer, Y. Leroy, J.M. Alonso, Jean Montreuil, Guy Ricart, Collin Colbert, D. Duquet, C. Dewaele, Bernard Fournet,

Metabolomics and Mass Spectrometry Studies

Conventional positive fast atom bombardment (FAB) and continuous-flow FAB analysis were carried out with permethylated lacto-N-tetraose. This latter method, a new approach, has been used to analyze a mixture of permethylated oligosaccharides by liquid chromatography-mass spectrometry (LC-MS) with a packed capillary fused-silica column and the continuous-flow probe as interface. Under these conditions, we found the continuous-flow probe to be superior to the conventional probe because its low matrix level increased the signal-to-noise ratio. The analysis of the mixture of permethylated oligosaccharide alditols obtained from hen ovomucoid by LC-MS using the continuous-flow probe as interface is described.