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Evaluation and Optimization of Different Cationic Liposome Formulations forin VivoGene Transfer

1996; Elsevier BV; Volume: 221; Issue: 1 Linguagem: Inglês

10.1006/bbrc.1996.0564

1090-2104

Nejat K. Egilmez, Yoshimi Iwanuma, Richard B. Bankert,

Virus-based gene therapy research

Five commonly used cationic liposome formulations were tested for their ability to deliver DNA to established subcutaneous human tumor xenografts in SCID mice. Liposomes were complexed with a mammalian expression plasmid containing the bacterial β-galactosidase gene and delivered to tumors by direct injection. The optimal lipid to DNA ratiosin vivowere markedly different than those observedin vitrofor each liposome formulation. Tumor size at the time of inoculation also effected transfection efficiency significantly. Of the five liposome formulations tested, DC-Cholesterol was found to be superior to all othersin vivo.Even under optimal conditions however, the efficiency ofin vivotransfection was low in our system (∼0.3%). Implications of these results forin vivogene therapy of tumors are discussed.