Off-line liquid chromatographic—mass spectrometric studies of o-phthalaldehyde-primary amine derivatives
1983; Elsevier BV; Volume: 261; Linguagem: Inglês
10.1016/s0021-9673(01)87967-x
ISSN1873-3778
AutoresRichard C. Simpson, Jimmy E. Spriggle, Hans Veening,
Tópico(s)Inorganic and Organometallic Chemistry
ResumoThe structures of the products formed between several n-aliphatic amines and o-phthalaldehyde (OPT) during routine post-column derivatization after high-performance liquid chromatographic (HPLC) elution, were determined by gas chromatography—mass spectrometry. OPT derivatization studies were carried out separately in the presence of mercaptoethanol (MCE) and in the presence of ethanethiol (ETH). Comparison of the mass spectra of the silylated OPT—MCE derivatized amines with those of the non-silylated OPT—ETH derivatives indicated a consistent fundamental structure with similar fragmentation patterns. It was shown that the structure of the fluorescent product obtained under analytical post-column HPLC derivatization conditions is a 1 -alkylthio-2-alkyl-substituted isoindole. This finding is in agreement with results reported earlier for preparative-scale reactions.
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