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Effects of salicylate, γ-resorcylate and gentisate on oxidase enzyme systems from guinea-pig liver mitochondria

1963; Elsevier BV; Volume: 12; Issue: 10 Linguagem: Inglês

10.1016/0006-2952(63)90085-6

1873-2968

W.J.W. Hines, Christopher Bryant, M. J. H. Smith,

Receptor Mechanisms and Signaling

The effects of salicylate, γ-resorcylate and gentisate on the oxidative activity of a particulate preparation from guinea-pig liver were studied. Low concentrations (0.1 mM) of gentisate markedly inhibited the succinate oxidase and succinate-cytochrome c reductase activities of the preparation. Considerably higher concentrations of salicylate and resorcylate were required to produce similar inhibitions of these enzyme systems. In high concentrations (50 mM) the three drugs inhibited succinate dehydrogenase and cytochrome oxidase activities. It was concluded that gentisate exerted a specific effect at some point on the electron chain between succinate dehydrogenase and cytochrome c. The high concentration of salicylate and resorcylate required for inhibition indicated a non-specific, “blanket” effect on several of the steps in the respiratory chain. Salicylate (10 mM), resorcylate (1 mM) and gentisate (0.1 mM) also inhibited the NADH-cytochrome c reductase activity of the preparation. The inhibitions due to salicylate and resorcylate, but not that caused by gentisate, were completely reversed by the addition of higher concentrations of NADH.