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Production of poly(3-hydroxybutyrate) from inexpensive substrates

2000; Elsevier BV; Volume: 27; Issue: 10 Linguagem: Inglês

10.1016/s0141-0229(00)00299-4

1879-0909

Beom Soo Kim,

Microbial Metabolic Engineering and Bioproduction

Two inexpensive substrates, starch and whey were used to produce poly(3-hydroxybutyrate) (PHB) in fed-batch cultures of Azotobacter chroococcum and recombinant Escherichia coli, respectively. Oxygen limitation increased PHB contents in both fermentations. In fed-batch culture of A. chroococcum, cell concentration of 54 g l(-1) with 46% PHB was obtained with oxygen limitation, whereas 71 g l(-1) of cell with 20% PHB was obtained without oxygen limitation. The timing of PHB biosynthesis in recombinant E. coli was controlled using the agitation speed of a stirred tank fermentor. A PHB content of 80% could be obtained with oxygen limitation by increasing the agitation speed up to only 500 rpm.