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Evaluation of liver weight changes following repeated administration of carbon tetrachloride in rats and body—liver weight relationship

1986; Elsevier BV; Volume: 40; Issue: 2 Linguagem: Inglês

10.1016/0300-483x(86)90077-6

1879-3185

Nobuo Uemitsu, Chihiro Nishimura, Hiroshi Nakayoshi,

Eicosanoids and Hypertension Pharmacology

The proper method of evaluating liver weight changes resulting from the primary toxic effect of a chemical was studied. We used a pair-feeding method with carbon tetrachloride (CCl4), a chemical known to cause an increase in liver weight. Male rats were assigned to an untreated control group, CCl4-treated groups (daily dose of 10 μl or 50 μl of CCl4 in olive oil/100 g body wt), olive oil-treated plus feed-restricted groups, and feed-restricted groups. In the 2 types of feed-restricted groups the body weight changes of the CCl4-treated groups were duplicated by imposing restrictions on feeding. Absolute and relative liver weights were compared among the 4 types of groups after treatment for 14 or 25 days. Either absolute or relative liver weight of the treated group when compared to that of the feed-restricted group was the most reliable and sensitive toxicity indicator. In comparing the treated group with the untreated control group, relative liver weight was a more sensitive toxicity indicator than absolute liver weight. However, when liver weight data for about 200 normal male rats were plotted against body weight, and the 95% confidence limits of the regression line (γ = ax) were estimated, most of the data for the feed-restricted rats were outside the lower confidence limit. This means that a comparison of the relative liver weights of the treated rats and untreated control rats is not strictly valid, because the body weight-liver weight relationships are not the same in the 2 groups.