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Buffy coat (top/bottom)‐ and whole‐blood filtration (top/top)‐produced red cell concentrates differ in size of extracellular vesicles

2015; Wiley; Volume: 109; Issue: 3 Linguagem: Inglês

10.1111/vox.12272

1423-0410

Beatriz Bicalho, Alberto dos Santos Pereira, Jason P. Acker,

Extracellular vesicles in disease

Background and Objectives The influence that blood component separation methods have on changes to the red blood cell membrane during storage is not well understood. In Canada, red cell concentrates ( RCC s) are produced using the buffy coat ( BC , top/bottom) and the whole‐blood filtration ( WBF , top/top) methods, and this study aimed at comparing their influence on the characteristics of the extracellular vesicles ( EV ) which accumulated in the respective products during storage. Materials and Methods Using flow cytometry, dynamic light scattering and mass spectrometry, we assessed RCC EV s for concentration, size, lipid composition and correlation with supernatant haemoglobin (Hb). Results Accumulation of RBC EV s ( CD 235a + ) with storage time was similar in WBF and BC RCC s. The size of the EV s changed from <100 nm at d5 to near 200 nm by d42, with the EV s from WBF being smaller ( P < 0·001) than BC RCC s at all storage times. The amount of EV ‐bound Hb in the WBF and BC units was similar (about 10% of total supernatant Hb). WBF EV s and BC EV s displayed similar lipid composition. Conclusion Haemolysis and EV s increase in BC and WBF RCC s during storage. Differences in the size characteristics of the EV s in WBF and BC RCC s suggest that non‐ RBC EV s are more prevalent in WBF products. Understanding the impact that manufacturing has on the characteristics of the different populations of EV s in RCC s will aid quality improvement efforts.