LH-RH-Stimulated gonadotropin release from the rainbow trout pituitary gland: An in vitro assay for detection of teleost gonadotropin releasing factor(s)
1980; Elsevier BV; Volume: 40; Issue: 3 Linguagem: Inglês
10.1016/0016-6480(80)90277-4
ISSN1095-6840
Autores Tópico(s)Ovarian function and disorders
ResumoPituitary glands collected from immature rainbow trout and incubated in vitro at 12° in Cortland's saline contained a low level of gonadotropic hormone (GtH). GtH released to the media was very low and not significantly increased by LH-RH treatment. Pituitaries from trout receiving testosterone treatment in vivo contained elevated amounts of GtH. A dramatic increase in pituitary GtH release in vitro was produced by LH-RH in doses down to 1 ng. Crude methanol:acetic acid extracts of a variety of fish hypothalami and the flounder forebrain contained GtH releasing factor activity. These data indicate that the GtH response of the steroid-treated immature trout pituitary gland provides a convenient method for detection of GtH releasing hormone substance(s) from the brain of both higher and lower vertebrates.
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