Production of Anti-Müllerian Hormone: Another Homology between Sertoli and Granulosa Cells
1984; Oxford University Press; Volume: 114; Issue: 4 Linguagem: Inglês
10.1210/endo-114-4-1315
ISSN1945-7170
AutoresB. Vigier, Jean‐Yves Picard, Dien Tran, Laurence Legeai‐Mallet, Nathalie Josso,
Tópico(s)Sperm and Testicular Function
ResumoAnti-Müllerian hormone (AMH) has been detected by RIA in the follicular fluid of mature bovine ovaries and in incubation medium of bovine granulosa cells. Purification of AMH from two independent batches of follicular fluid was achieved with a yield of 11% and 15% respectively. Both ovarian and control testicular AMH produced near-complete regression of fetal rat Müllerian ducts exposed to it in culture at a final concentration of 200-300 mU/ml and were recognized by the same monoclonal and polyclonal antibodies. These findings indicate that adult mammalian granulosa cells are capable of producing immunoreactive and bioactive AMH at a rate apparently similar to that already demonstrated for mature Sertoli cells and add yet another item to the homologies reported between male and female somatic gonadal cells.
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