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Hydrogen Sulfide Mediated Inhibitory Neurotransmission to the Pig Bladder Neck: Role of K ATP Channels, Sensory Nerves and Calcium Signaling

2013; Lippincott Williams & Wilkins; Volume: 190; Issue: 2 Linguagem: Inglês

10.1016/j.juro.2013.02.103

1527-3792

Vítor S. Fernandes, Ana S. F. Ribeiro, María Victoria Barahona, Luís M. Orensanz, Ana Martínez‐Sáenz, Paz Recio, Ana Cristina Martı́nez, Salvador Bustamante, Joaquı́n Carballido, Albino Garcı́a-Sacristán, Dolores Prieto, Medardo Hernández,

Sulfur Compounds in Biology

No AccessJournal of UrologyInvestigative Urology1 Aug 2013Hydrogen Sulfide Mediated Inhibitory Neurotransmission to the Pig Bladder Neck: Role of KATP Channels, Sensory Nerves and Calcium Signaling Vítor S. Fernandes, Ana S.F. Ribeiro, María Victoria Barahona, Luis M. Orensanz, Ana Martínez-Sáenz, Paz Recio, Ana Cristina Martínez, Salvador Bustamante, Joaquín Carballido, Albino García-Sacristán, Dolores Prieto, and Medardo Hernández Vítor S. FernandesVítor S. Fernandes Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain , Ana S.F. RibeiroAna S.F. Ribeiro Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain , María Victoria BarahonaMaría Victoria Barahona Departamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain , Luis M. OrensanzLuis M. Orensanz Departamento de Investigación, Hospital Universitario Ramón y Cajal, Madrid, Spain , Ana Martínez-SáenzAna Martínez-Sáenz Departamento de Urología, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain , Paz RecioPaz Recio Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain , Ana Cristina MartínezAna Cristina Martínez Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain , Salvador BustamanteSalvador Bustamante Departamento de Urología, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain , Joaquín CarballidoJoaquín Carballido Departamento de Urología, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain , Albino García-SacristánAlbino García-Sacristán Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain , Dolores PrietoDolores Prieto Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain , and Medardo HernándezMedardo Hernández Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.103AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Because neuronal released endogenous H2S has a key role in relaxation of the bladder outflow region, we investigated the mechanisms involved in H2S dependent inhibitory neurotransmission to the pig bladder neck. Materials and Methods: Bladder neck strips were mounted in myographs for isometric force recording and simultaneous measurement of intracellular Ca2+ and tension. Results: On phenylephrine contracted preparations electrical field stimulation and the H2S donor GYY4137 evoked frequency and concentration dependent relaxation, which was reduced by desensitizing capsaicin sensitive primary afferents with capsaicin, and the blockade of adenosine 5′-triphosphate dependent K+ channels, cyclooxygenase and cyclooxygenase-1 with glibenclamide, indomethacin and SC560, respectively. Inhibition of vanilloid, transient receptor potential A1, transient receptor potential vanilloid 1, vasoactive intestinal peptide/pituitary adenylyl cyclase-activating polypeptide and calcitonin gene-related peptide receptors with capsazepine, HC030031, AMG9810, PACAP6-38 and CGRP8-37, respectively, also decreased electrical field stimulation and GYY4137 responses. H2S relaxation was not changed by guanylyl cyclase, protein kinase A, or Ca2+ activated or voltage gated K+ channel inhibitors. GYY4137 inhibited the contractions induced by phenylephrine and by K+ enriched (80 mM) physiological saline solution. To a lesser extent it decreased the phenylephrine and K+ induced increases in intracellular Ca2+. Conclusions: H2S produces pig bladder neck relaxation via activation of adenosine 5′-triphosphate dependent K+ channel and by smooth muscle intracellular Ca2+ desensitization dependent mechanisms. H2S also promotes the release of sensory neuropeptides and cyclooxygenase-1 pathway derived prostanoids from capsaicin sensitive primary afferents via transient receptor potential A1, transient receptor potential vanilloid 1 and/or related ion channel activation. References 1 : Two's company, three's a crowd: can H2S be the third endogenous gaseous transmitter?. FASEB J2002; 16: 1792. 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Google Scholar © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 190Issue 2August 2013Page: 746-756 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.Keywordsurinary bladdermuscle, smoothpotassium channelshydrogen sulfidesynaptic transmissionAcknowledgmentsFrancisco Puente and Manuel Perales provided technical assistance. Industrias Cárnicas Vaquero, Madrid, Spain, provided the bladders.MetricsAuthor Information Vítor S. Fernandes Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Ana S.F. Ribeiro Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author María Victoria Barahona Departamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Luis M. Orensanz Departamento de Investigación, Hospital Universitario Ramón y Cajal, Madrid, Spain More articles by this author Ana Martínez-Sáenz Departamento de Urología, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain More articles by this author Paz Recio Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Ana Cristina Martínez Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Salvador Bustamante Departamento de Urología, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain More articles by this author Joaquín Carballido Departamento de Urología, Hospital Universitario Puerta de Hierro Majadahonda, Madrid, Spain More articles by this author Albino García-Sacristán Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Dolores Prieto Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Medardo Hernández Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain More articles by this author Expand All Advertisement PDF downloadLoading ...