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Mechanism of the mutagenic action of hydroxylamine

1965; Elsevier BV; Volume: 11; Issue: 4 Linguagem: Inglês

10.1016/s0022-2836(65)80024-9

1089-8638

D. M. Brown, John H. Phillips,

Chemical Reactions and Isotopes

The reaction of aqueous hydroxylamine (pH 6·5) with polycytidylic acid has been studied by comparing at intervals the loss of ultraviolet light absorption (a measure of the addition of the reagent to the 4,5-double bond) and the ratio of cytidylic to N6-hydroxycytidylic acid formed after acid hydrolysis. An initial lag in the production of N6-hydroxycytidylic acid is taken, with earlier chemical evidence, to indicate that the primary action of the reagent leads to the formation of 4-hydroxylamino-4,5-dihydrocytosine residues. The latter species is considered to be responsible for replication error in hydroxylamine mutagenesis.